Fig. 4
From: The immunomodulator decoy receptor 3 improves locomotor functional recovery after spinal cord injury
DcR3 treatment increased the expression of anti-inflammatory cytokines. Five millimeters of the spinal cord tissue from the lesion sites of DcR3.Fc-treated and control SCI rats was extracted on day 7 post-injury. Anti-inflammatory associated cytokines, namely IL-4, IL-10, and IL-13, were analyzed by qPCR. The target gene quantity was normalized to a reference gene (GAPDH) using the following formula: 2−(Ct(target) − Ct(reference)). The expression of anti-inflammatory cytokines, including IL-4 and IL-10, was significantly elevated at the lesion sites of DcR3-treated SCI rats (**p < 0.01, DcR3.Fc, N = 5; control, N = 6). The results are presented as means ± SEM