Fig. 2

Inflammatory profile in the brain of ROCV-infected BALB/c mice. a Number of CD3⁻ cells in the brain after 5 or 7 days of infection with ROCV 1.10⁶ PFU analyzed by FACS. b Number of CD3⁺ cells after ROCV infection as indicated. c Number of CD4⁺- and CD8⁺-infiltrating cells in the brain after 7 days post-infection (disease peak) with ROCV 1.10⁶ PFU, gated on CD3⁺ cells. d Expression levels of TNF-α mRNA in the brain of mice infected with ROCV 1.10⁶ PFU 5 and 7 days post-infection, analyzed by qPCR. e qPCR of IFN-γ mRNA expression in the brains of ROCV-infected mice. f IL-17A mRNA expression. *p < 0.05, **p < 0.001 or ***p < 0.0001. NS non-significant. Data are representative as the mean of three independent experiments with at least four animals per group. Statistical analysis was performed by Student t test using the software GraphPad Prism