Fig. 3

IL-33 expression in CNS-infiltrating cells. a Frequency of F4/80⁺ cells in the CNS of false infected WT mouse (WT mock), analyzed by FACS. Frequency of F4/80⁺ cells isolated from 1.10⁶ PFU ROCV-infected mouse 7 days after infection. (WT 7 days p.i.) Expression of IL-33 on F4/80⁺ gated cells isolated from CNS of mock and 1.10⁶ PFU ROCV-infected mouse, analyzed by flow cytometry—FACS. Dots are representative from a single animal (with at least three animals per group). b, c Expression levels of IL-33 and ST2L (transmembrane form) mRNA in the brain of mice infected with ROCV 1.10⁶ PFU, analyzed by qPCR. Data are representative as the mean of three independent experiments with at least four animals per group. Statistical analysis was performed by unpaired Student t test using the software GraphPad Prism