Fig. 6

IL-33 and nitric oxide participates on ROCV-induced encephalitis. a iNOS mRNA expression in the brain of WT, ST2^−/−- and STAT6^−/−-infected mice. b Arg1 mRNA expression in the brain of WT,ST2^-/- and STAT6-/- infected and control mice. mRNA expression was determined with qPCR normalized for the GAPDH CT value. Data are expressed as mean ± SE. c Frequency of F4/80+ cells (gated on CD45+) isolated from brain tissues of WT, ST2^−/− and STAT6^−/− 9 days after infection with ROCV. d iNOS expression (gated on CD45+ F4/80+ cells). e Mean fluorescence intensity (MFI) of iNOS+ cells. f Groups of ten female 6-week-old WT or iNOS-deficient mice (iNOS^−/−) were infected intraperitoneally with 1.106 PFU of ROCV and monitored daily for survival up to 21 days post-infection. g Clinical score of ROCV-infected mice. Mice were infected with 1.106 PFU of ROCV and sacrificed as indicated. *p < 0.05, **p < 0.001. Statistical analysis was performed by Student t test using the software GraphPad Prism. Data are representative as the mean of three independent experiments with at least four animals per group