Fig. 6

Neutralizing CCL20 decreases the expression of pro-inflammatory cytokines. a The qRT-PCR data at 24 h post-injury shows that treatment with neutralizing anti-CCL20 antibody significantly decreased mRNA expression of IL-1β, IL-6, and TNF-α. However, CCL20 mAb had no effect on the expression of the anti-inflammatory IL-10. b Similarly, ELISA data at 24 h post-injury illustrates that CCL20 blockade significantly reduced protein expression of IL-1β, IL-6, and TNF-α but had no influence on the expression of IL-10. ⁺ P < 0.05, compared with the sham group; ^* P < 0.05, compared with the CCL20 mAb group