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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Infiltrating T lymphocytes reduce myeloid phagocytosis activity in synucleinopathy model

Fig. 1

Increased numbers of α-synuclein in the presence of lymphocytes. a, b Representative bright field pictures of the a substantia nigra and b striatum show α-synuclein aggregates in WTS+ Rag2+/+ and WTS+ Rag2−/− but not in WTS mouse brains. α-synuclein was stained in the brain tissue of 22–26-week-old mice using anti-α-synuclein (15G7) antibody. Scale bars 100 μm. Arrows indicate α-synuclein aggregates determined as brown spots of at least 3 μm in diameter and are shown by arrows. c, d Significantly reduced numbers of α-synuclein aggregates were found in WTS+ Rag2−/− compared to WTS+ Rag2+/+ mice in both the substantia nigra and striatum without and with proteinase K (PK) treatment. Control mice (WTS) did not show any aggregates. T-test **p < 0.01, ***p < 0.001. e The protein level of α-synuclein was determined by western blotting indicating increased amount of α-synuclein protein in WTS+ Rag2+/+ mice compared to WTS+ Rag2−/− mice (n = 3 per group). f WTS+ Rag2+/+ and WTS+ Rag2−/− brains were stained for the dopaminergic neuronal marker tyrosine hydroxylase (TH), and representative pictures of the substantia nigra are shown. g No significant difference in the number of TH+ neurons could be detected by stereological quantification in the SN of WTS+ Rag2+/+ compared to WTS+ Rag2−/− mice. Scale bar 50 μm. c, d, g Data from nine to ten (five for WTS) mice/group are shown

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