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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Infiltrating T lymphocytes reduce myeloid phagocytosis activity in synucleinopathy model

Fig. 2

CD3+ lymphocytes infiltrate the midbrain of WTS+ Rag2+/+ mice. a WTS+ Rag2+/+ and WTS+ Rag2−/− as well as WTS brains were stained for the T lymphocyte marker CD3 as shown in representative bright field pictures of the midbrain and the quantification in b. Infiltrating CD3+ lymphocytes, labeled with asterisks, do not co-localize with blood vessels (indicated by the dotted lines). CD3+ cells were only present in WTS+ Rag2+/+ mice, but not in WTS+ Rag2−/− or WTS mice. Scale bar 50 μm. b Data from five mice/group are shown. c–e Flow cytometry analysis of the brain tissues was performed to confirm the presence and to analyze the subsets of T lymphocytes in WTS+ Rag2+/+ mice. c 30,000 events were recorded, and a total population was defined in the forward/side scatter, excluding dead cells, which were identified by utilizing a live-dead staining, for further gating strategies (upper histogram). The CD4+ (CD11b) population representing CD4+ T lymphocytes and distinguished from CD4 low positive CD11b+ microglia population is shown in a representative dot plot (lower histogram). Representative scatter and dot plot are shown from brain tissue of a WTS+ Rag2+/+ mouse. d Gated populations for the T lymphocyte subset markers CD4 (T helper cell) and CD8 (cytotoxic T cell) are visualized in representative dot plots, and total cell numbers of CD4 and CD8 T cells per group ± SEM are shown in e, which were determined based on bead measurement and calculation of the total cell number. Significantly more CD4+ and CD8+ T cells are present in WTS+ Rag2+/+ compared to WTS+ Rag2−/− and WTS mouse brain tissues. Data from five mice/group are shown. T-test, *p < 0.05, ***p < 0.001

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