Fig. 3

Increased presence of infiltrating myeloid cells in brains of WTS+ Rag2^−/− mice. a Representative fluorescence pictures of the substantia nigra (SN) of WTS⁺ Rag2^+/+ and WTS⁺ Rag2^−/− mice stained for TH⁺ neurons to visualize the SN (upper panel) and for Iba1 to visualize CNS myeloid cells in the SN (lower panel). Scale bars 50 μm. The total number of b Iba1⁺ myeloid cells in the SN, c CD11b mRNA expression measured by qRT-PCR and d CD11b⁺ CD45^lo cell frequency measured by flow cytometry (total of 30,000 recorded events, total brain cell population was defined as shown in Fig. 2c, defined as shown in the representative dot plots in e, red box) were unchanged between WTS⁺ Rag2^+/+ and WTS⁺ Rag2^−/− mouse brain tissues. e, f A significant increase in infiltrating myeloid cells defined as CD11b⁺ CD45^hi population (green box in E) was found in WTS⁺ Rag2^−/− compared to WTS⁺ Rag2^+/+ brains in flow cytometry analysis. Data from four to five mice/group are shown as means ± SEM. T-test, **p < 0.01