Fig. 4

Infiltrating T lymphocytes are associated with a M1 myeloid phenotype. a Gene expression of characteristic M1 markers (TNF-α, IL-1β, ICAM1) measured by qRT-PCR shows their upregulation in WTS⁺ Rag2^+/+ mice compared to WTS⁺ Rag2^−/− mice, while genes associated with an anti-inflammatory M2 phenotype (Arg1, Lgals3, CD200r) are upregulated in WTS⁺ Rag2^−/− compared to WTS⁺ Rag2^+/+ mice. The gene expression values in WTS⁺ mice were normalized to the respective gene expression levels in WTS⁻ mice. RNA was extracted from the whole-brain lysates. Data are shown as mean of five mice/group ± SEM. b The phagocytic receptor, CD206, is increased in brain tissue of WTS⁺ Rag2^−/− compared to WTS⁺ Rag2^+/+ mice on protein level as shown by western blot analysis with three mice/group (upper panel) and by densitometric quantification (lower panel) Data are presented as mean ± SEM