Fig. 5

T lymphocytes reduce phagocytosis activity of microglia in vitro. a BV2 microglia cells were co-cultivated with aggregated α-synuclein (200nM) in absence or presence of T or B lymphocytes at the 10:1 ratio (BV2/lymphocytes) for 24 h. b Recombinant human α-synuclein was aggregated in vitro (electron microscopy picture, scale bar 0.2 μm). c α-synuclein uptake was quantified as percentage of Iba1 and α-synuclein double-positive cells within total Iba1+ population and normalized to a control uptake level in the absence of lymphocytes. α-synuclein uptake was significantly decreased in the presence of T cells, but not B cells, compared to control condition. d Co-culture of BV2 cells under the same conditions as in c with T lymphocytes isolated from WTS⁻ mice did not alter the α-synuclein uptake compared to the co-culture with T lymphocytes from WTS⁺ mice. Scale bar 20 μm. T-test, *p < 0.05, ***p < 0.001