Fig. 5

Heatmap of CSF and serum AQP4-antibody levels against AQP4-M23, AQP4-M1, and AQP4-M23 mutants (columns) in baseline samples of 6 NMOSD patients with paired CSF and serum samples. Rows are individual samples with patient IDs (Nr), flow cytometry AQP4-M23 binding ratios (BR), and AQP4-IgG binding patterns (BP) shown at the right side. Data are shown as percent binding of AQP4-M23. Values range from blue (0 %) to white (50 %) to red (100 %). Columns were clustered according to their Pearson’s correlation coefficients and rows were cluster according to their Eucledian distance (both average linkage). Two major antibody binding patterns were identified, a loop A-dependent pattern A and an independent pattern B. Five of the six patients had different antibody recognition patterns in CSF and serum. The heatmap was generated using GENE-E matrix visualization and analysis software (http://www.broadinstitute.org/cancer/software/GENE-E/index.html)