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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Gas1 up-regulation is inducible and contributes to cell apoptosis in reactive astrocytes in the substantia nigra of LPS and MPTP models

Fig. 4

Influence of LPS, IFNγ, TNFα, and their combinations on expression of Gas1 and iNOS, cell viability, and cell death, and generation of reactive oxygen species (ROS) in cell culture of astrocytes. a Gas1-immunoreactivity in astrocytes after treatment with LPS, IFNγ, and TNFα. b Immunoblots of Gas1 and iNOS in the astrocytes with distinct treatments at 24 h. c Comparison of Gas1 and iNOS levels among above groups. d Cell viability determined by CCK-8 assay. e Cell death determined by LDH release assay. f ROS generation assay in above distinct groups of astrocytes. ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001 vs control (mean ± S.E.M., n = 3)

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