Fig. 4

Differences in transcriptomic profiles (cytokines, chemokines, and interferon response genes) between established BV2 cell lines and PM. a Heat map representation depicting the common expression of positive regulators of inflammatory genes between BV2 cell lines and PM cells after 2- and 4-h LPS stimulation. b Heat map representation of the positive regulators of inflammatory transcripts that were unique to PM cells, which showed a distinct signature after 2- and 4-h LPS stimulation compared to BV2 cell lines. c UCSC Browser images representing normalized RNA-seq read densities. d Transcript abundance (in read count) was evaluated using RNA-seq in 2- and 4-h LPS-induced BV2 cell lines and PM cells. e Quantitative real-time reverse transcriptase-PCR analysis of LPS-induced positive regulators of inflammatory gene expression (cytokines, chemokines, and interferon response genes) that were common and unique to PM compared to BV2 cell lines. Gene expression was normalized to the GAPDH transcript levels. *P < 0.01 and **P < 0.001 compared to the control. The data represent three biologically independent experiments