Fig. 5

Differences in the expression of selected TF families between BV2 cell lines and PM. a Heat map representation showing the commonly expressed TF families between BV2 cell lines and PM cells after 2- and 4-h LPS stimulation. b Heat map of the TF families that were unique to PM cells, which showed a distinct signature following 2- and 4-h LPS stimulation compared to BV2 cell lines. c UCSC Browser images representing normalized RNA-seq read densities. d Transcript abundance (in read count) was evaluated using RNA-seq in 2- and 4-h LPS-induced BV2 cell lines and PM cells. e Confirmation of differentially expressed TFs was performed using quantitative reverse transcription-polymerase chain reaction. The genes that were common and unique to PM cells compared to BV2 cell lines are shown. Gene expression was normalized to GAPDH transcript levels. *P < 0.01 and **P < 0.001 compared to the control. The data represent three biologically independent experiments. f Patterns of TF motif enrichment within the promoters of the indicated genes in 4-h LPS-induced PM cells. g The activity of highly connected positive regulators of the inflammatory genes IRF1, IRF2, STAT1, and STAT2 led to the activation of this network, as assessed using the IPA molecule activity predictor in LPS-induced PM cells. h Results of the GO term analysis using DAVID. The genes that were regulated by STAT1 and IRF1 in response to LPS in PM cells are shown