Fig. 1
Prostaglandin E2 and other activators of cAMP production increase PKA activity in sensory neuronal cultures. a Each point represents mean ± SEM of PKA activity normalized to total PKA after 10-min exposure to various concentrations of PGE2 from 4 to 6 independent harvests of cells maintained in the absence of added NGF. Asterisks indicate a statistically significant increase in PKA activity compared to the vehicle-treated control using one-way ANOVA followed by Bonferroni’s post hoc test. b Each column represents the mean ± SEM of PKA activity normalized to total PKA after a 10-min exposure to vehicle (V), PGE2, the EP4 receptor agonist, L902688, cPGI2, forskolin, CTX, or isoproterenol (Iso) as indicated. An asterisk indicates a statistically significant difference between PKA activation by each treatment compared to its respective vehicle control using Student’s t test