Fig. 2

URMC-099 inhibits Aβ-activated microglial pro-inflammatory cytokines. a A conventional RT2-qPCR was performed to measure IL-1β, IL-6, or TNF-α expression using primer sets (Table 1) and synthesized cDNA with total RNA isolated from murine microglia (n = 3 per group). b Quantification of IL-1β, IL-6, or TNF-α protein secretion from murine microglia (n = 3 per group). Data are presented as mean ± SEM, ^a,c,d p < 0.05, ^aa,cc,dd p < 0.01, ^aaa p < 0.001, ^avs control, ^cvs URMC-099, ^dvs Aβ + URMC-099, one-way ANOVA, Newman–Keuls post hoc test