Fig. 4

Fluoro-Jade C staining of the deep cerebellar white matter (“arbor vitae”). a Fixed, frozen 30-μm sections from the cerebellum were stained with Fluoro-jade C. Low-powered images show intense staining of the deep cerebellar white matter (wm) with minimal punctate staining of the granule layer (gr). There was no staining of Purkinje cells (p) or within the molecular layer (m). High-powered images were photographed from within the white matter (wm) labeled with an asterisk. Arrows represent the borders of the deep white matter of the cerebellum. b Quantification of Fluoro-Jade C intensity was performed on 10 images from three animals per experimental group (30 images per condition). The mean densitometric fluorescence intensity was calculated using ImageJ software, and groups were compared using the student’s t test. *p ≤ 0.05; ***p ≤0.001