Fig. 2

Inhibitory effects of ASH-WEX and FIV on LPS-induced inflammatory mediators’ expression in primary microglia. A Representative confocal images of activated primary microglial cells with or without pretreatment with ASH-WEX and FIV immunostained with (a) NFkB and (b) AP1. B i, ii Histograms represent the relative optical intensities of NFkB and AP1, respectively, in different treated groups as compared to control. C i Represents the relative production of the nitrite in different treated groups as compared to control due to neuroinflammation as estimated by the Griess reagent. C ii Representative MMP zymograms for control and different treated groups and histograms represent densitometric analysis of MMP bands. *p < 0.05 represents statistically significant difference between control and LPS + ASH-WEX and LPS + FIV-treated groups. ⁺ p < 0.05 represents statistically significant difference between control and LPS-treated group. ^# p < 0.05 represents statistically significant difference between LPS and LPS + ASH-WEX and LPS + FIV-treated groups