Fig. 3

Inhibition of CXCL13 by shRNA lentivirus attenuated pIONL-induced mechanical allodynia. a Pretreatment with LV-Cxcl13 shRNA increased the contact time between 3 and 21 days after the operation, compared to LV-NC-injected mice. Intra-TG injection was performed 7 days before pIONL (arrow). *P < 0.05, **P < 0.01, ***P < 0.001. Two-way repeated measures ANOVA followed by Bonferroni test. b Posttreatment with LV-Cxcl13 shRNA increased the contact time between 7 and 14 days after the operation, compared to LV-NC treatment. Intra-TG injection was performed 3 days after pIONL (arrow). *P < 0.05, **P < 0.01. Two-way repeated measures ANOVA followed by Bonferroni test. c Representative fluorescence photomicrograph shows GFP expression in the TG 7 days after intra-TG infusion of lentivirus vector. d Real-time PCR assay of Cxcl13 shows that pretreatment with LV-Cxcl13 shRNA inhibited pIONL-induced Cxcl13 upregulation. **P < 0.01, ***P < 0.001. Student’s t test