Fig. 7

pIONL-induced mechanical allodynia is mediated by ERK-dependent proinflammatory cytokines production in the TG. a pIONL increased TNF-α and IL-1β expression 10 days after pIONL in WT mice, but not in Cxcr5 KO mice. *P < 0.05, ***P < 0.001. One-way ANOVA followed by Bonferroni test. b Intra-TG injection of MEK inhibitor, PD98059 10 days after pIONL attenuated pIONL-induced mechanical allodynia at 3 h. **P < 0.01. Two-way repeated measures ANOVA followed by Bonferroni test. The same treatment reduced the expression of TNF-α (c) and IL-1β (d). **P < 0.01, Student’s t test. e Intra-TG injection of TNF-α inhibitor, Etanercept 10 days after pIONL alleviated pIONL-induced mechanical allodynia. The effect was shown 1 h after injection and maintained for more than 6 h. *P < 0.05, ***P < 0.001. Two-way repeated measures ANOVA followed by Bonferroni test. f Intra-TG injection of IL-1β inhibitor, Diacerein 10 days after pIONL alleviated pIONL-induced mechanical allodynia from 1 to 6 h. *P < 0.05, ***P < 0.001. Two-way repeated measures ANOVA followed by Bonferroni test