Fig. 3
From: TLR2 and TLR9 modulate enteric nervous system inflammatory responses to lipopolysaccharide
MAMP stimulation induces activation of the NF-kB pathway in embryonic ENS culture. a Rat embryonic ENS culture was incubated for 8 h with the indicated MAMPs or the IkB phosphorylation inhibitor Bay 11-7082, and cell protein extraction and Western blot were performed to determine phosphorylated IkB (P-IkBα). B-actin was used as a loading control. b Time-course densitometric quantification of P-IkBα bands corrected to β-actin and related to basal activation levels; representative bands are shown in Additional file 4. Statistical analysis was performed independently for each ligand, using one-way ANOVA followed by Dunnett’s test (n = 4 for each ligand and time point; *P < 0.05, **P < 0.01 and ***P < 0.001). c Representative micrographs showing NF-kB p65 subunit localisation in basal conditions (Ctrl) or 1 h after LPS challenge (LPS). White arrows point to neuronal nuclei displaying p65 positive staining. Scale bars: 25 μm