Fig. 2
CCR5 regulates JE progression by altering the infiltration of leukocytes in the brain. a, b The frequency and number of Ly-6Chi monocytes and Ly-6Ghi granulocytes in the brain. Ccr5+/+ and Ccr5−/− mice were inoculated i.p. with JEV (3.0 × 107 pfu), and the frequency (a) and total number (b) of Ly-6Chi monocytes and Ly-6Ghi granulocytes in the CNS were determined by flow cytometric analysis at 3, 5, and 7 dpi using vigorous heart perfusion. Values in representative dot-plots denote the average percentage of the indicated population after gating on CD11b+ cells. c, d Resting and activated microglia/macrophage number in the CNS. The number of resting (CD11c−CD11bhiCD45intF4/80+) and activated (CD11c-CD11bhiCD45hi F4/80+) as well as other myeloid-derived leukocytes (CD11bintCD45hi F4/80+) was enumerated using flow cytometric analysis at 3 dpi. Values in representative dot-plots denote the average percentage of the indicated population after gating on CD11c−F4/80+ cells. e Accumulated number of NK cells, CD4+, and CD8+ T cells in the CNS. Total accumulated number of NK cells (CD3−NK1.1+DX5+), CD4+ (CD3+CD4+), and CD8+ (CD3+CD8+) T cells in the CNS were enumerated using flow cytometric analysis at 3, 5, and 7 dpi. Data are averages ± SD of the indicated cell populations derived from at least three independent experiments (n = 4–5). *p < 0.05; **p < 0.01; ***p < 0.001 compared with the levels of the indicated groups