Fig. 4
Kinetic analysis of DC subpopulations, myeloid cells, NK cells, and CD4/CD8 T cells in the spleen and blood of CCR5-ablated mice. a–c Kinetic analysis of DC subpopulations, Ly-6Chi monocytes, Ly-6Ghi neutrophils, and NK cells in the spleen. Ccr5+/+ and Ccr5−/− mice were inoculated i.p. with JEV (3.0 × 107 pfu), and the absolute numbers of splenic DC subpopulations (CD11c+CD11b+ myeloid DC and CD11c+CD8α+ lymphoid DC) (a), Ly-6Chi monocytes and Ly-6Ghi neutrophils (b), and NK cells (c) were determined using flow cytometric analysis at the indicated time points. d Activation of NK cells. The activation of CD3−NK1.1+DX5+ NK cells was evaluated by intracellular IFN-γ staining upon stimulation with PMA plus ionomycin. Values in the histograms on the left denote the average percentages of IFN-γ-producing cells after gating on CD3−NK1.1+DX5+ NK cells. e Absolute number of CD4+ and CD8+ T cells in the spleen. Splenic CD4+ and CD8+ T cells were enumerated from 1 to 7 dpi. f–h Kinetic analysis of myeloid, NK, and T cells in the blood of Ccr5+/+ and Ccr5−/− mice. The numbers of myeloid cells (Ly-6Chi monocytes and Ly-6Ghi neutrophils) (f), NK cells (g), and CD4+/CD8+ T cells (h) were determined using flow cytometric analysis at the indicated time points. Data are averages ± SD of values derived from at least three independent experiments (n = 3–5). *p < 0.05; **p < 0.01; ***p < 0.001 compared to the levels in the corresponding groups