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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: CCR5 ameliorates Japanese encephalitis via dictating the equilibrium of regulatory CD4+Foxp3+ T and IL-17+CD4+ Th17 cells

Fig. 7

CCR5+CD4+Foxp3+ Tregs ameliorate JE in CCR5-ablated mice. a Susceptibility of CCR5-ablated mice to JE following adoptive transfer of CCR5+CD4+Foxp3+ Tregs. CCR5+CD4+Foxp3+ and CCR5−CD4+Foxp3+ Treg cells were purified from Ccr5+/+.Foxp3GFP and Ccr5−/−.Foxp3GFP mice and adoptively transferred to Ccr5−/− mice (n = 10) at 2 days following JEV (3.0 × 107 pfu) infection, respectively. Surviving recipient mice were examined daily. Ccr5+/+ and Ccr5−/− mice that did not receive Tregs were used as positive and negative controls, respectively. b Ratio of mice showing neurological disorders in CCR5+ Treg-injected CCR5-ablated mice. Ccr5−/− recipients that received CCR5+ or CCR5− Tregs were examined every 6 h from 4 to 11 dpi. c Changes in body weight of CCR5+ Treg-injected CCR5-ablated mice during JE. Data are averages ± SD of body weight relative to the time of challenge. d Viral burden in lymphoid tissue and CNS of Treg-injected Ccr5−/− recipient. Viral burden in the spleen, brain, and spinal cord of Ccr5−/− recipients was assessed by real-time qRT-PCR at 5 dpi. The viral RNA load was expressed as viral RNA copy number per microgram of total RNA. e, f The expression of pro- and anti-inflammatory cytokines in lymphoid tissue and CNS of Treg-injected Ccr5−/− recipients. The expression of pro- and anti-inflammatory cytokines in the brain (e) and spleen (f) of Treg-injected Ccr5−/− recipients was determined by real-time qRT-PCR at 5 dpi. Data are averages ± SD of values derived from at least three independent experiments (n = 5–6). *p < 0.05; **p < 0.01 compared with the levels of the indicated groups

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