CCR5 ameliorates Japanese encephalitis via dictating the equilibrium of regulatory CD4+Foxp3+ T and IL-17+CD4+ Th17 cells

Kim, Jin Hyoung; Patil, Ajit Mahadev; Choi, Jin Young; Kim, Seong Bum; Uyangaa, Erdenebelig; Hossain, Ferdaus Mohd Altaf; Park, Sang-Youel; Lee, John Hwa; Eo, Seong Kug

doi:10.1186/s12974-016-0656-x

Journal of Neuroinflammation

Table 1 Specific primers for the expression of cytokines, chemokines, transcription factor, and JEV RNA used in real-time qRT-PCR

From: CCR5 ameliorates Japanese encephalitis via dictating the equilibrium of regulatory CD4⁺Foxp3⁺ T and IL-17⁺CD4⁺ Th17 cells

Gene name	Primer sequence (5′-3′)	Position cDNA	Gene bank ID
IL-1β	FP: AAGTGATATTCTCCATGAGCTTTGT	535–559	NM_008361
IL-1β	RP: TTCTTCTTTGGGTATTGCTTGG	679–700	NM_008361
IL-6	FP: TGG GAA ATC GTG GAA ATG AG	209–228	NM_031168
IL-6	RP: CTC TGA AGG ACT CTG GCT TTG	442–462	NM_031168
IL-10	FP: CAA CAT ACT GCT AAC CGA CTC CT	253–275	NM_010548
IL-10	RP: TGA GGG TCT TCA GCT TCT CAC	405–425	NM_010548
IL-17	FP: TCT GAT GCT GTT GCT GCT G	87–105	NM_010552.3
IL-17	RP: ACG GTT AGA GGT AGT CTG AGG	254–267	NM_010552.3
IFN-γ	FP: CAG CAA CAA CAT AAG CGT CA	119–220	NM_008337.3
IFN-γ	RP: CCT CAA ACT TGG CAA TAC TCA	119–220	NM_008337.3
CCL2	FP: AAA AAC CTG GAT CGG AAC CAA	347–367	NM_011333
CCL2	RP: CGG GTC AAC TTC ACA TTC AAA G	426–447	NM_011333
CCL3	FP: CCA AGT CTT CTC AGC GCC AT	158–177	NM_011337.2
CCL3	RP: GAA TCT TCC GGC TGT AGG AGA AG	206–228	NM_011337.2
CCL4	FP: TTC TGT GCT CCA GGG TTC TC	128–147	NM_013652.2
CCL4	RP: GAG GAG GCC TCT CCT GAA GT	388–407	NM_013652.2
CCL5	FP: CCC TCA CCA TCA TCC TCA CT	77–96	NM_013653.3
CCL5	RP: CTT CTT CTC TGG GTT GGC AC	275–294	NM_013653.3
CXCL1	FP: CGC TGC TGC TGC TGG CCA CC	101–120	NM_008176.3
CXCL1	RP: GGC TAT GAC TTG GGT TTG GG	245–264	NM_008176.3
CXCL2	FP: ATC CAG AGC TTG AGT GTG ACG C	194–215	NM_009140.2
CXCL2	RP: AAG GCA AAC TTT TTG ACC GC	264–283	NM_009140.2
FOXP3	FP: GGC CCT TCT CCA GGA CAG A	551–570	NM_054039.2
FOXP3	RP: GCT GAT CAT GGC TGG GTT GT	642–662	NM_054039.2
GATA3	FP: AGT CCT CAT CTC TTC ACC TTC C	1027–1048	NM_008091.3
GATA3	RP: GGC ACT CTT TCT CAT CTT GCC TG	1116–1138	NM_008091.3
RORγt	FP: CCG CTG AGA GGG CTT CAC	75–93	AJ1232394
RORγt	RP: TGC AGG AGT AGG CCA CAT TAC	283–304	AJ1232394
T-bet	FP: GCC AGG GAA CCG CTT ATA TG	823–843	AF241242
T-bet	RP: GAC GAT CAT CTG GGT CAC ATT GT	935–958	AF241242
JEV	FP: GGC TTA GCG CTC ACA TCC A	4132–4150	AB920399.1
JEV	RP: GCT GGC CAC CCT CTC TTC TT	4207–4226	AB920399.1
β-actin	FP: TGG AAT CCT GTG GCA TCC ATG AAA C	885–909	NM_007393.3
β-actin	RP: TAA AAC GCA GCT CAG TAA CAG TCC G	1209–1233	NM_007393.3

IL interleukin, FP forward primer, RP reverse primer

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ISSN: 1742-2094

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