Skip to main content
Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: SPARC expression by cerebral microvascular endothelial cells in vitro and its influence on blood-brain barrier properties

Fig. 3

a, b Growth media serum concentration influences SPARC expression in confluent hCMEC/D3 cells. SPARC protein levels were quantified by western blot analysis of lysates derived from cultures propagated in media with varied serum concentration. Relative SPARC protein levels were normalized to GAPDH. SPARC detected in lysates resolved at the molecular weight observed for rh-SPARC (~43 kDa). Bars represent the average of four immunoblots, two independent experiments (exps. A and B) performed in duplicate. Error bars indicate SEM. One-way ANOVA and Tukey’s multiple comparison test, *P < 0.05

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094