Fig. 5

Clemastine modulates the activation pattern of SOD1-G93A spinal microglia cultures from symptomatic mice. Spinal primary microglia from SOD1-G93A mice at PND 120 are exposed to clemastine 30 μM for 6 h. a Equal amounts of total lysates are subjected to western blotting with anti-CD68, anti-ARG-1, anti-CD163, anti-P2X7, anti-P2X4, anti-P2Y12 and anti-GAPDH for protein normalisation. Data represent mean ± S.E.M. of n = 3 independent experiments. Statistical significance is calculated by student’s t test referred to untreated cells (Ctrl), * p < 0.05. Cells are then analysed by means of a confocal microscope (scale bar 20 μm) after staining with anti-CD11b (red) and in b with anti-CD68 (green), in c with anti-ARG-1 (green), in d with anti-CD163 (green) and in e with anti-P2Y12 (green)