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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Actions of the antihistaminergic clemastine on presymptomatic SOD1-G93A mice ameliorate ALS disease progression

Fig. 7

Autophagy is modulated by clemastine in a time-dependent manner in NSC34 SOD1-G93A motor neuron cells. NSC34 SOD1-G93A motor neurons are exposed to clemastine 30 μM for 6 h. Equal amounts of treated cells lysates are subjected to western blotting with anti-LC3I/II (a), anti-SQSTM1/p62 (b), anti-mTOR and anti-p-mTOR (c) or anti-SOD1 (d). Anti-GAPDH is used for protein normalisation. NSC34 SOD1-G93A motor neurons are exposed to clemastine 30 μM for 24 h. Equal amounts of treated cells lysates are subjected to western blotting with anti-LC3I/II (e), anti-SQSTM1/p62 (f), anti-mTOR and anti-p-mTOR (g) or anti-SOD1 (h). Anti-GAPDH was used for protein normalisation. Data represent mean ± S.E.M. of n = 3 independent experiments. Statistical significance is calculated by student’s t test referred to untreated cells (Ctrl), * p < 0.05

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