Fig. 2From: Fractalkine shedding is mediated by p38 and the ADAM10 protease under pro-inflammatory conditions in human astrocytesIL-1β, TNF-α and IFN-γ induce CX3CL1 mRNA synthesis in human astrocytes. a Experimental timeline and treatments are shown. Human astrocytes were serum starved for 3 h prior to stimulation with b, e IL-1β (100 pg/ml), c, f TNF-α (10 ng/ml) or d IFN-γ (10 ng/ml) for either 3 h (grown in standard media) or 18 h (grown in speciality media). All graphs show significant increase in CX3CL1 mRNA levels after 3 and 18 h. Data presented as mean ± SEM, n = 4–8, unpaired t test, *p < 0.05, ***p < 0.001 vs. corresponding controlBack to article page