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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Fractalkine shedding is mediated by p38 and the ADAM10 protease under pro-inflammatory conditions in human astrocytes

Fig. 6

IKKβ inhibitor attenuates sCX3CL1 release. Human astrocytes (grown in speciality media) were serum starved for 3 h and pre-treated with the NF-kB inhibitor, TPCA-1 (IKKβ inhib.; 3, 1, 0.3, 0.1 and 0.03 μM), for 30 min. Cells were then stimulated with either IL-1β (100 pg/ml) or TNF-α (10 ng/ml) for 18 h. Quantification of CX3CL1 ELISA revealed a concentration-dependent decrease in sCX3CL1 release for both IL-1β- and TNF-α-treated groups. ###p < 0.001 compared to own control; **p < 0.01 and ***p < 0.001 compared to the matched treated group. Values expressed as averages ± SEM; n = 3 each condition done in duplicate. (one-way ANOVA and Tukey’s post hoc test)

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