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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Class I PI3K inhibitor ZSTK474 mediates a shift in microglial/macrophage phenotype and inhibits inflammatory response in mice with cerebral ischemia/reperfusion injury

Fig. 2

ZSTK474 inhibits the expression of Iba-1 and GFAP at 72 h after cerebral reperfusion injury. GFAP and Iba-1 are specific markers of microglia/macrophages and astrocytes. a, b The number of microglia/ macrophages and astrocytes increased as demonstrated by immunofluorescent staining. However, ZSTK474 administration decreased the number of Iba-1-stained and GFAP-stained cells. c, d Quantification of microglial/macrophage and astrocyte numbers of A, B. e, f Protein levels of Iba-1 were dramatically lower in the ZSTK474-treated group, as demonstrated by Western blotting. Magnification: ×20 in A and B. Data in graphs are expressed as means ± SD; # P < 0.001, *P < 0.05, vs. control group; n = 3

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