Table 2 Immunohistochemistry: primary antibodies
From: Dysregulation of the (immuno)proteasome pathway in malformations of cortical development
Antigen | Primary antibody | Source | Dilution |
|---|---|---|---|
Glial fibrillary acidic protein (GFAP) | Rabbit polyclonal | DAKO, Glostrup, Denmark | 1:4000 |
Neuronal nuclear protein (NeuN) | Mouse clone MAB377 | Chemicon, Temecula, CA, USA | 1:2000 |
Phospho-S6 ribosomal protein (pS6) | Ser235/236; rabbit polyclonal | Cell Signaling Technology, Beverly, MA, USA | 1:50 |
Interleukin 1β | Goat polyclonal | Santa Cruz Bio., Delaware CA, USA | 1:70 |
MHC class I (HLA A, B, and C; HLA-I) | Mouse clone HC-10 | a | 1:200 |
MHC class II (HLA-DP, DQ, DR; HLA-II) | Mouse clone CR3/43 | DAKO, Glostrup, Denmark | 1:400 |
Proteasome β1 | Mouse monoclonal IgG1 | Enzo Life Sciences/Biomol, Farmingdale, NY, USA | 1:200 |
Proteasome β5 | Rabbit polyclonal | Enzo Life Sciences/Biomol | 1:500 |
Proteasome β1i | Mouse monoclonal IgG1 | Enzo Life Sciences/Biomol | 1:200 |
Proteasome β5i | Mouse monoclonal IgG1 | Enzo Life Sciences/Biomol | 1:200 |