Fig. 6

TBEV infection results in the induction of RANTES promoter activity. a T98G cells were co-transfected with pGL2-220 and pRL-TK, followed by UV-inactivated TBEV or TBEV infection at different MOIs; 24 h p.i. cells were harvested to measure luciferase activity. The results are expressed as fold induction of RANTES promoter activity relative to the basal level. *P < 0.05 versus mock control. b T98G cells were co-transfected with pGL2-220 and pRL-TK, followed by TBEV infection at an MOI of 0.1. At indicated times p.i., cells were harvested to measure luciferase activity. The results are expressed as fold induction of RANTES promoter activity relative to the basal level. *P < 0.05 versus mock control. c Schematic representation of the RANTES promoter constructs [29]. Locations of the putative binding sites for CRE, ISRE, NF-IL-6, and NF-kB are illustrated. Numbering is relative to the transcription initiation site. d Effect of site mutations in the RANTES promoter sequence on TBEV-inducible activity. T98G cells were transiently transfected with site-mutated (Mut) plasmids of the pGL2-220 RANTES promoter and infected with TBEV for 36 h. The results are expressed as fold induction of RANTES promoter activity relative to the basal level. Bars represent the means ± the standard deviations of three independent experiments. *P < 0.05 compared with pGL2-220 plus TBEV infection