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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: High-fat diet feeding differentially affects the development of inflammation in the central nervous system

Fig. 3

Activation state of glial cells in the cortex (CTX) and the cerebellum (CBL) at week 16. a Representative photomicrographs of the cerebellum and cortex immunostained for microglial cells with Iba-1. b Quantification of the area occupied by microglial cells in the cortex and the cerebellum of CTL (white column) and HFD (black column) mice at week 16. Microglial cells were identified using immunohistology in both CNS structures with Iba-1. mRNA expression of c CD11b and d CD11c, both myeloid lineage markers in the cortex and cerebellum of HFD mice at 16 weeks. e Representative photomicrographs of the cortex and cerebellum immunostained for astrocytes with GFAP. f Quantification of the area occupied by astrocytes in the cerebellum and the cortex of CTL (white column) and HFD (black column) mice at week 16. Astrocytes were identified using immunohistology in both CNS structures with GFAP. mRNA expression of g GFAP and h Serpina3n (an astrogliosis marker) in the cortex and the cerebellum of CTL and HFD mice at week 16. Data are mean ± s.e.m. The standard diet groups were set at 100% for area quantification and at 1 for mRNA expression. The white columns represent mice fed a standard diet and the black columns represent the mice fed a HFD. One-way ANOVA with Bonferroni’s post test or Kruskal-Wallis test with Dunn’s post test between HFD group and its respective CTL group (*P < 0.05) and between CNS regions (#P < 0.05 and ##P < 0.01). Scale bar 50 μm

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