Table 1 Primer sequences
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
CD11b | GAACATCCCATGACCTTCCA | GCTGGGGGACAGTAGAAACA |
CD11c | ACGTCAGTACAAGGAGATGTTGGA | ATCCTATTGCAGAATGCTTCTTTACC |
CD68 | CTTCCCACAGGCAGCACAG | AATGATGAGAGGCAGCAAGAGG |
Claudin 1 | TTCGCAAAGCACCGGGCAGATACA | GCCACTAATGTCGCCAGACCTGAAA |
Claudin 5 | GTTAAGGCACGGGTAGCACT | GTACTTCTGTGACACCGGCA |
COX-2 | TGACCCCCAAGGCTCAAATAT | TGAACCCAGGTCCTCGCTTA |
F4/80 | TGACAACCAGACGGCTTGTG | CAGGCGAGGAAAAGATAG |
GFAP | TTCGCACTCAATACGAGGCA | CTCCAGATCGCAGGTCAAG |
IL-1β | TCGCTCAGGGTCACAAGAAA | CATCAGAGGCAAGGAGGAAAAC |
IL-6 | ACAAGTCGGAGGCTTAATTACACAT | TTGCCATTGCACAACTCTTTTC |
iNOS | AGGTACTCAGCGTGCTCCAC | GCACCGAAGATATCTTCATG |
LBP | AGTCCTGGGAATCTGTCCTTG | ACTTGTGCCTTGTCTGGATG |
MCP-1 | GCAGTTAACGCCCCACTCA | TCCAGCCTACTCATTGGGATCA |
Occludin | ATGTCCGGCCGATGCTCTC | TTTGGCTGCTCTTGGGTCTGTAT |
RPL19 | TGACCTGGATGAGAAGGATGAG | CTGTGATACATATGGCGGTCAATC |
Serpina3n | GGACATTGATGGTGCTGGTGAAT | CTCCTCTTGCCCGCGTAGAA |
TNFα | CCACCACGCTCTTCTGTCT | TCCAGCTGCTCCTCCACTT |
ZO-1 | TTTTTGACAGGGGGAGTGG | TGCTGCAGAGGTCAAAGTTCAAG |