Fig. 2

Cytokine-primed NSCs hinder cell cycle progression in LNC in vitro. Unfractionated LNC were co-cultured with NSC for 72 h as in Fig. 1. EdU was added to the cells for the last 5 h of culture. Cells were then recovered, labelled with anti-CD4 mAb, stained for intracellular EdU content, and analysed by flow cytometry. Events are depicted after gating on CD4⁺ T cells. Relative fractions of EdU⁺ cells are represented. Data are expressed as mean EdU fold suppression (±SD) calculated by dividing the percent of EdU⁺CD44^high cells over the control CD4⁺ T cells from n ≥ 3 independent experiments. Unpaired two-tailed t test for comparisons between groups were applied; **p ≤ 0.01 and ***p ≤ 0.001 vs. LNC; °p ≤ 0.05 and °°p ≤ 0.01 vs. NSCs/LNC