Fig. 3
From: Metabolic determinants of the immune modulatory function of neural stem cells
Untargeted metabolomics analysis of NSCs under Th1- and Th2-like cytokines: Multivariate analysis (PLS-DA) and hierarchical clustering. a PLS-DA score assessing the clustering of the three sample groups (basal NSCs (group 0), Th1 NSCs (group 1), or Th2 NSCs (group 2)) was performed on significant metabolites by using MetaboAnalyst 2.0 software. b Hierarchical clustering heatmap for MS peak intensities of significantly (p ≤ 0.05) different intracellular metabolites among basal NSCs, Th1 NSCs, or Th2 NSCs. MultiExperiment Viewer software (MeV) (v 4.6.2) was applied on NSCs significant endometabolome dataset with Pearson correlation metric. Sixty-one intracellular metabolites were significantly different in the three experimental conditions among 215 identified metabolites by LC-MS/MS and GC-MS/MS