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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Colony-stimulating factor 1 receptor blockade prevents fractionated whole-brain irradiation-induced memory deficits

Fig. 4

CSF-1R blockade reduces blood monocytes and prevents monocyte accumulation in the brain after radiation. a Gating strategy used in flow cytometry analyses for blood samples. b Comparison of blood monocyte between control and PLX5622-treated animals before irradiation (**p < 0.01, n = 8, t test). c Analyses of blood monocyte 3 days after the last radiation fraction (*p < 0.05; FPLX5622 = 24.97, p < 0.0001; FIRR = 0.004, p = 0.95; FInteraction = 0.007, p = 0.93; n = 6, two-way ANOVA). d Gating strategy used in flow cytometry analyses for brain samples. e Comparison of monocyte population in the brain between control and PLX5622-treated groups before irradiation (n = 8). f Analyses of brain monocytes 3 days after the last radiation fraction (**p < 0.01, ns = not significant, FPLX5622 = 9.334, p = 0.0045; FIRR = 7.079, p = 0.0121; FInteraction = 9.336, p = 0.0045; n = 9, two-way ANOVA). g Gating used to distinguish microglia from infiltrating myeloid cells (CD45-low vs CD45-high populations) (h) Comparison of microglia numbers before irradiation (***p < 0.001, n = 8, t test). i Analyses of microglia numbers 3 days after the last radiation fraction (*p < 0.05, **p < 0.01, FPLX5622 = 37.54, p = 0.0001; FIRR = 1.602, p = 0.234; FInteraction = 0.3323, p = 0.577; n = 9, two-way ANOVA)

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