Fig. 10

Acute stress exposure increases proliferation of microglia in numerous brain regions of Cx3cr1 ^wt/gfp mice. Representative confocal images show that PCNA+ CX3CR1-GFP+ microglia are more prevalent in the medial prefrontal cortex (a) and dorsal hippocampus (c) of mice exposed to acute stress. b, d Comparable regions from chronic SD mice. Inset panels between (a) and (b) provide magnified views of PCNA colocalization with GFP microglia and demonstrate the distribution of proliferating cells within cortical layers (I, II, III, IV, and V). Proliferating cells within the rostral migratory stream (RMS) are provided for contrast. Insets below c show PCNA+ CX3CR1-GFP+ microglia within dentate gyrus and surrounding area. Arrows indicate CX3CR1-GFP+ microglia colabeled with PCNA. e Example of CX3CR1-GFP+ microglia colabeled with PCNA is shown. Scale bars: a and b = 200 μm (insets = 20 μm); c and d = 100 μm (c insets = 10 μm); e = 20 μm. LV lateral ventricle. A summary of group differences is provided in Table 3; n = 8 per group