Fig. 3

Stress does not cause extravasation of Ccr2 ^wt/rfp macrophages (RFP, red) into the brain parenchyma. a Representative bivariate dot plot showing gating strategy for RFP in a HC control Ccr2 ^wt/rfp mouse. b Bivariate dot plot showing CX3CR1-GFP+ microglia, CCR2-RFP+ macrophages, and CCR2-RFP CX3CR1-GFP dual positive cells in the brains of CCR2 ^wt/rfp CX3CR1 ^wt/gfp mice exposed to HC and acute (ASD) and chronic social defeat (CSD). c Average number of CCR2-RFP+ cells and CCR2-RFP+ CX3CR1-GFP+ double-positive cells detected in all conditions (mean ± SEM n = 6). d Peripheral CCR2-RFP+ macrophages are detected in the choroid plexus of CCR2 ^wt/rfp CX3CR1 ^wt/gfp mice, regardless of stress exposure. HC condition is shown. The red peripheral CCR2-RFP+ cells contrast with green CX3CR1-GFP+ microglia. e Magnified view of choroid plexus detailing RFP/GFP dual positive cells with arrows. f Peripheral CCR2-RFP+ macrophages (red) are detected in the leptomeninges but not in the brain parenchyma of the dorsal hippocampus. ChP choroid plexus. Six animals per group were examined. Scale bars: d – f = 100 μm