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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Social defeat induces depressive-like states and microglial activation without involvement of peripheral macrophages

Fig. 5

GFP+ splenocytes transferred from UBC-GFP mice do not extravasate into the brain of stressed wildtype mice. The experimental timeline is shown in (a) for positive control conditions and experimental conditions. Transferred GFP+ cells colonize the spleen of wildtype recipient (example is from a mouse in chronic SD) (b). Representative bivariate dot plots of brain cells isolated from wildtype naïve and from wildtype mice with adoptive transfer of UBC splenocytes (c). Cells are stained and gated for CD11bhi cells and UBC-GFP+ cells. UBC-GFP+ cells are observed in the brains of mice that received inflammatory stimulus (LPS + 1L-1β) but not in experimental stress conditions. d Flow cytometry confirmed the presence of UBC-GFP+ cells in the spleens of wildtype mice receiving transfer of cells from Ubc gfp/gfp donors. (n = 4 per group). Scale bar = 20 μm

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