Fig. 6
Microglia show regionally specific morphological changes to LPS, but not to SD stress in CX3CR1WT/gfp mice. a Panel provides examples of four morphological parameters used to characterize the shape of segmented cells: soma size, cell processes spread, eccentricity, and cell perimeter length. Initial image is provided for comparison. Scale bar = 20 μm. b Graphs show relative changes in microglia morphometric parameters in the prefrontal cortex (PFC), hippocampus (HIPP), and paraventricular nucleus (PVN) of the hypothalamus of mice exposed to acute SD (ASD), chronic SD (CSD), or 2 mg/kg LPS. Significant changes occur only in mice exposed to LPS, and these effects are region-dependent. For each parameter, values represent mean fold change ± SEM compared to HC (n = 10 for HC, ASD, and CSD groups, and n = 6 for the LPS group). One-way ANOVA followed by Bonferroni’s post hoc test was used for each morphometric parameter in each area. Bonferroni correction for multiple comparisons has been applied. *** Indicate statistical significance compared to all other groups p < 0.001