Fig. 4
From: Fractalkine suppression during hepatic encephalopathy promotes neuroinflammation in mice
Microglia activation in AOM-treated mice was reduced by ICV infusion of soluble fractalkine. a Representative immunoblot images and quantification of pERK1/2 and tERK1/2 in cortex lysates from vehicle or AOM-treated mice infused ICV with saline or soluble fractalkine (sFKN). b Z-stack confocal images of individual microglia stained with IBA1 (red). Microglia when activated become more amoeboid shaped with retracted cellular processes. c Field staining for IBA1 (red) in the cortex of vehicle or AOM-treated mice infused ICV with saline or sFKN. d Quantification of IBA1 field staining in the cortex of vehicle or AOM-treated mice infused with saline or sFKN. For quantitative IBA1 immunofluorescence analyses, *p < 0.05 compared to vehicle-treated mice infused with saline, # p < 0.05 compared to AOM-treated mice infused with saline. n = 4 for immunoblot analyses and n = 10 for IBA1 field quantification analysis