Fig. 3

Epitopes of human PNS autoantibodies at the node of Ranvier. There are two isoforms of neurofascin (NF). a The neuronal isoform NF 186 (NF186, O94856) that resides within the node. b The glial isoform NF 155 (NF155, O94856) that resides on the myelin loop within the paranode. Anti-NF186 antibodies target the extracellular fibronectin type III (Fn) V (or Fn5) and Mucin-like domain. Anti-NF155 antibodies target extracellular domains Fn III (or Fn3) and Fn IV (Fn4) [107]. c Antibodies directed against neuronal contactin (Q12860) target N-glycosylation sites N467, N473, and N494, within the extracellular immunoglobulin (Ig) C2-like domain 5 [108]. Human protein topology, i.e., aa sequences and transmembrane domains (TM), is adapted from UniProt database, and UniProt identifiers are shown between brackets. Diagrams do not depict protein crystal structure. Dark green highlights major epitopes mapped by methods which retain the native in vivo conformational structure of the protein, such as cell-based assays (Table 1)