Fig. 2

IL-1ra preventive treatment improves EAE behavioral syndrome and restores striatal CB1R function. a–a” At the LDT, the IL-1ra treatment improved the behavioral alterations of EAE mice; the time spent in the light zone, EAE-IL-1ra mice showed values similar to CFA-vehicle, although being not significantly different to both CFA and EAE-vehicle (a). a’ Vertical activity increased in EAE-IL-ra mice, indicating an ameliorated explorative response induced by IL1-ra treatment. Examples of LDT video-tracking are depicted in a”. b, b’ Consistently with the results obtained in non-minipump-implanted animals (shown in Fig. 1b, b ’), the nesting score of EAE-vehicle mice was worse than CFA-vehicle animals and IL-1ra icv treatment corrected these behavioral alterations (b). Representative photographs in b’. c, c’. The lack of the depressant effect mediated by HU210 in EAE slices was rescued in EAE mice receiving in vivo treatment of IL-1ra by minipump implantation (c). In c’, there are examples of electrophysiological traces showing the lack of the effect of HU210 only in EAE-vehicle mice. Values are means ± SEM. Statistical differences were analyzed by one-way ANOVA for multiple comparisons (followed by Tukey HSD or Dunn's comparisons) or by unpaired T-test for rear numbers in LDT. *p < 0.05, **p < 0.01, ***p < 0.001