Fig. 3

CB1R function is linked to dopamine system in the EAE striatum. a The inhibitory effect of HU210 on sIPSC frequency of striatal neurons was completely restored in EAE mice receiving i.p. injection of amphetamine (p < 0.05). b–b” Amphetamine treatment did not affect DARPP32 phosphorylation in the striatum of control CFA mice, as shown by the WB image in b and the densitometric analysis in b’. The treatment did not change the amount of unphosphorylated protein, as shown in b and b”. c–c” Representative WB of striatal protein extracts from EAE-vehicle and EAE-amphetamine mice: the densitometric analysis of the bands displays an upregulation of Th34-DARPP32 in EAE-vehicle samples and a partial recovery of such phosphorylation in EAE-amphetamine group, as shown by graph in c’. Amphetamine treatment induced a significant upregulation of DARPP32 expression in EAE striatum (c”). WB data are normalized to CFA values. Values are means ± SEM. Statistical differences were analyzed by paired Student’s T test (electrophysiology) and one-way ANOVA followed by Tukey HSD (WB). *p < 0.05 EAE-amphetamine vs EAE-vehicle