Fig. 4

miR-17-92 cluster regulates astrocytogenesis and neurogenesis of NSCs in vitro. a, b Immunofluorescence labeling of NSCs showing the expression of astrocyte marker GFAP or neuron marker doublecortin (DCX) after infecting with lentivirus overexpressing miR-17-92 cluster (miR-17-92) or lentivirus expressing EGFP alone (CON) in the different differential media. Arrow heads indicate double positive cells. Scale bar, 50 μm. c, d Percentage of GFAP-positive or DCX-positive among total EGFP-positive cells from the experiment shown in a or b was determined. Values are means ± SEM. Asterisks indicate a statistically significant difference compared with the control group (*P < 0.05, unpaired two-tailed t test, n = 3 independent experiments)