Fig. 5

PH3 and nestin immunoreactivity in grafted NSCs. a Schematic presentation of temporal course of transplantation, immunohistochemical test, and behavioral analysis. b Diagram illustrating the injury site (red line) in the motor cortex (MC) and NSC grafting (green dot). c Immunofluorescence labeling of coronal sections for the mitotic marker PH3 (red), 1 day after transplantation of either control (CON-NSC) or miR-17-92-overexpressing (miR-17-92-NSC) cells (green). No positive signal was observed in the grafted cells. Scale bar, 50 μm. d, f Immunofluorescence labeling of coronal sections for the NSC marker nestin (red), 1 day and 1 week after transplantation of either CON-NSC or miR-17-92-NSC (green). Both types of grafted cells express nestin at 1 day, while no positive signal was observed at 7 days. Scale bar, 50 μm. e The ratio of nestin-positive to EGFP-positive cells from the experiment shown in d was determined. There was no statistically significant difference between the two groups (N.S. not significant, unpaired two-tailed t test, n = 3 independent experiments)