Fig. 3

Attenuated microgliosis and reduced lymphocyte infiltration characterizes the striatum of EAE mice treated with siponimod. a Double immunostaining of coronal striatal sections (in gray DAPI nuclei) showing expression of Iba1-positive microglia/macrophage cells (red) and of CD3 (green) in EAE-siponimod and EAE-vehicle mice. Only few lymphocytes could be detected in the striatum of mice treated with siponimod, and microgliosis is visibly reduced in the same animals. Scale bar: 100 μm. b The levels of IBA1 and CD3 mRNA were quantified by qRT-PCR in the striatum of EAE-siponimod versus EAE-vehicle mice using β-actin as internal control. The histogram shows that both IBA1 and CD3 mRNA were significantly reduced in the striatum of siponimod-treated mice. Statistical significance was evaluated by unpaired T test: *p < 0.05