Fig. 6

Brain infusion of siponimod promotes the survival of PV+ GABAergic interneurons during the course of EAE. a Low magnification images (×5 objective) of coronal striatal sections from control CFA-vehicle, EAE-vehicle, and EAE-siponimod showing area selected for PV counting on serial sections (dorsal striatum) and DAB-immunolabeled interneurons (scale bar: 200 μm): at high magnification, the density of PV+ cells is visibly reduced in EAE-vehicle compared to that in CFA-vehicle with a partial recovery in EAE-siponimod slices. Yellow arrows indicate PV-labeled cells (scale bar: 20 μm). b Histogram represents the quantitative stereological counts of PV+ cells performed on striatal slices: the intracerebroventricular treatment with siponimod significantly increases the total number of PV+ cells in the dorsal left striatum of EAE mice. Data are expressed as mean ± S.E.M. Statistical comparisons showed in the graph were calculated with Newman-Keuls post hoc: CFA-vehicle vs EAE-vehicle **p < 0.01; CFA-vehicle vs EAE-siponimod p > 0.05; EAE-vehicle vs EAE-siponimod*p < 0.05